Prevalence and characterization of carbapenemases producing gram-negative bacteria among clinical isolates in a tertiary care super speciality hospital in South India
Author(s): Jaya Banerjee, N Pavani and Rajkumar Rathod
Abstract: Background: Antimicrobial resistance (AMR) poses a universal threat to health care systems, which could result in deaths. The present study aims to find the prevalence of different carbapenemases, the number of carbapenemases expression (single or multiple) and their genetic background among carbapenem resistant isolates (
Enterobacterales, Pseudomonas and
Acinetobacter strains).
Materials and Methods: Current observational, prospective study is conducted for the period of 4 years. All clinical samples of both inpatient and outpatient departments were cultured in the clinical microbiology department. Demographic details of patients were collected from the Hospital Information System. Identification of isolates and antibiotic susceptibility testing was performed on automated Vitek-2 compact system. Carbapenem resistance was decided on the basis of minimum inhibitory concentration (MIC) values as determined by VITEK system and interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guide lines. A total of 445 non-duplicate Carbapenem resistant isolates were further subjected to X-pert Carba-R Real Time Multiplex PCR Assay to detect the type of carbapenemases expressed.
Results: Out of 445 samples collected 70.78% were from males and 29.2% were from females with an age range of 15 to 87years. Approximately 96.62 % of the isolated carbapenem resistant strains were carbapenemase producers. Multiple carbapenemase gene detected in 64% isolates, co-production of
blaNDM and blaOXA-48 genes was evidenced in 56.4% isolates. Among all, 24% were positive for blaNDM, 11.23% for blaOXA-48 and 0.44% for blaVIM.
K. pneumoniae was the most common carbapenemase gene producer (74.3%), followed by
Pseudomonas aeruginosa (13.2%) and
E. coli (6.06%). 51.23% of the
K. pneumoniae isolates had blaNDM and blaOXA-48 simultaneously. Co-production of blaNDM and blaVIM in one isolate and blaNDM and blaKPC in two isolates of
Pseudomonas aeruginosa were identified.
Conclusion: K. pneumoniae, E. coli, Pseudomonas aeruginosa, Acinetobacter baumannii were the most common strains isolated among the different specimens.
DOI: 10.22271/27069567.2025.v7.i4a.674Pages: 04-09 | Views: 96 | Downloads: 27Download Full Article: Click Here
How to cite this article:
Jaya Banerjee, N Pavani, Rajkumar Rathod.
Prevalence and characterization of carbapenemases producing gram-negative bacteria among clinical isolates in a tertiary care super speciality hospital in South India. Int J Adv Res Med 2025;7(4):04-09. DOI:
10.22271/27069567.2025.v7.i4a.674
