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International Journal of Advanced Research in Medicine
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2024, Vol. 6, Issue 3, Part A

Metastasis of cervical cancer cell line affected by probiotics in real-time PCR assay


Author(s): Azher Adnan and Zainab M Farhan

Abstract:
Objective: The term "probiotic" refers to live microorganisms that have been linked to advantageous outcomes for the host. Nonetheless, a large body of research suggests that probiotic lactic acid bacteria (LAB) may be helpful in preventing colon cancer in its early stages. However, nothing is known about how probiotic LAB may affect colorectal and cervical cancer in their later phases, particularly with regard to metastasis. The present research sought to examine the impact of Lactobacillus crispatus supernatant (LCS) on cervical cell lines derived from human tissue, specifically focusing on the HeLa cell line. Throughout our inquiry, this was going to be executed.
Materials and Methods: Using the MTT test, the experiment's activities impact of the LCS was ascertained. Through the utilization of a quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), an investigation into the expression of the MMP2 and MMP9 genes was carried out after the cells had been synchronized.
Results: Probiotics called Lactobacillus crispatus supernatant cytotoxically affected HeLa cells. Furthermore, LCS did not affect normal cells adversely. Additionally, it was demonstrated that treatment with the lactobacilli under study resulted in a decrease in the expression of the MMP2 and MMP9 genes. Our research indicates that LCS effectively inhibits metastatic potency in HeLa cells by down regulating MMP2 and MMP9 expression while up regulating the expression of their inhibitors.
Conclusion: We have shown that a probiotic can delay the progression of late-stage cancer illness and has anti-metastasis effects on cell lines. LCS exhibited both cytotoxic and anti-metastatic effects on HeLa cell lines. HeLa cervical cancer cells are resistant to proliferation when exposed to L. crispatus. It is required to conduct additional evaluations in order to assess our findings on the other cancer cell lines before considering the use of these probiotics in larger trial studies.



DOI: 10.22271/27069567.2024.v6.i3a.570

Pages: 48-55 | Views: 306 | Downloads: 113

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International Journal of Advanced Research in Medicine
How to cite this article:
Azher Adnan, Zainab M Farhan. Metastasis of cervical cancer cell line affected by probiotics in real-time PCR assay. Int J Adv Res Med 2024;6(3):48-55. DOI: 10.22271/27069567.2024.v6.i3a.570
International Journal of Advanced Research in Medicine
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