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International Journal of Advanced Research in Medicine
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2024, Vol. 6, Issue 3, Part A

Detection of MRSA and MSSA biofilms in clinical specimens from a tertiary care hospital located in Hyderabad


Author(s): Pavani Chitthaluru, Venugopal Kura, Kanduri Prashanthi and Galla Jhansi Rani

Abstract: Background: MRSA, or methicillin-resistant Staphylococcus aureus, is a common pathogen that causes a wide range of infections, ranging from minor skin infections to severe conditions such as endocarditis and osteoarticular infections, which are associated with significant morbidity and mortality rates. Biofilm formation is crucial in MRSA's capacity to infiltrate, propagate, and withstand antimicrobial therapies.
Methods: This study was performed at the microbiology division of a tertiary healthcare facility in Hyderabad. The identification of MRSA was carried out using the cefoxitin disk diffusion method. At the same time, the detection of biofilm was performed through the utilization of microtiter plate and Congo red agar techniques.
Results: In this study, 235 samples of S. aureus were analyzed. Of these, 104 samples were identified as MRSA, and the remaining 131 were classified as MSSA (Methicillin-Susceptible Staphylococcus aureus)). Seventy of the 104 MRSA (67%) samples and sixty-two of the 131 MSSA (47%) samples were found to be multidrug-resistant, and these samples were checked for biofilm production in this study. According to the microtiter plate method, MRSA showed 26% of the biofilm production, while only 4.3% was found on Congo Red Agar. Comparatively, the Congo red agar method demonstrated just 2% and the microtiter plate method demonstrated 10% of biofilm formation in MSSA.
Conclusion: This study shows that, when compared to Congo red agar, the Microtiter plate method is more accurate for detecting biofilm-producing staphylococci. Compared to MSSA strains, MRSA strains typically produced more biofilm. Biofilm producer exhibits broad antibiotic resistance. Before administering treatment, clinicians, along with the microbiologists, should routinely monitor biofilm formation in hospitals. To enhance biofilm management in healthcare settings, educating stakeholders about biofilms and ad hoc efficacy tests is crucial, which are frequently academic.


DOI: 10.22271/27069567.2024.v6.i3a.563

Pages: 15-21 | Views: 276 | Downloads: 113

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International Journal of Advanced Research in Medicine
How to cite this article:
Pavani Chitthaluru, Venugopal Kura, Kanduri Prashanthi, Galla Jhansi Rani. Detection of MRSA and MSSA biofilms in clinical specimens from a tertiary care hospital located in Hyderabad. Int J Adv Res Med 2024;6(3):15-21. DOI: 10.22271/27069567.2024.v6.i3a.563
International Journal of Advanced Research in Medicine
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